《植物生理学报》 2018, 54(10): 1561-1568

火龙果开花调控转录因子基因HpSOC1的克隆与表达分析

严佳文, 解璞, 袁启凤, 肖图舰, 毛永亚, 马玉华^*

贵州省农业科学院果树科学研究所, 贵阳550006

通信作者：马玉华；E-mail: myh79@163.com

摘　要：

SOC1是拟南芥成花诱导过程中的关键基因之一, 其表达产物能整合多条成花途径的调控信号。为揭示火龙果(Hylocereus polyrhizus) SOC1同源基因在光周期成花途径中的作用机制, 以火龙果芽为试验材料, 应用RACE技术克隆了SOC1同源基因, 命名为HpSOC1。其cDNA全长为1 365 bp, 开放阅读框长度为651 bp, 编码216个氨基酸, GenBank登录号为MH230063。HpSOC1蛋白具有典型的MADS-box、K-box结构域和SOC1-MOTIF, 属于MADS基因家族中的SOC1/TM3亚家族, 无信号肽输出位点和跨膜螺旋区, 定位于细胞核内, 属于非分泌蛋白。基因表达分析结果显示, HpSOC1在茎、茎芽和花芽中的表达量显著高于根、花瓣和果皮; 相比对照, 光照处理8 d时, HpSOC1表达显著上调。推测HpSOC1可能在营养生长向生殖生长转变的过程中发挥作用。

关键词：火龙果; 光周期; SOC1同源基因; 克隆; 基因表达

收稿：2018-08-06   修定：2018-09-30

资助：贵州省重点科技攻关项目(黔科合NZ[2015]3001号)、公益性行业(农业)科研专项(201403036)、贵州省改革转制项目(黔科合体Z字[2014]4003号)和贵州省科技计划项目(黔科合平台人才[2017]5603)。

Molecular cloning and expression analysis of the flowering regulating transcription factor HpSOC1 gene in Hylocereus polyrhizus

YAN Jia-Wen, XIE Pu, YUAN Qi-Feng, XIAO Tu-Jian, MAO Yong-Ya, MA Yu-Hua^*

Institute of Pomology, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China

Corresponding author: MA Yu-Hua; E-mail: myh79@163.com

Abstract:

SOC1 is one of the key genes in flowering induction of Arabidopsis thaliana, and its encoded protein can integrate regulatory signals of multiple flowering pathways. To explore the molecular mechanism of SOC1 homologous gene in photoperiod flowering pathway of pitaya [Hylocereus polyrhizus (Weber) Britton & Rose], HpSOC1 was cloned through rapid amplification of cDNA ends technique from the bud of a pitaya strain qianmilong. The full-length cDNA and open reading frame (ORF) of HpSOC1 is 1 365 bp and 651 bp, respectively, could encode 216 amino acids, and the GenBank accession number is MH230063. Sequence alignment and motif analysis showed that the deduced amino acids contains typical MADS-box and K-box domain, and a highly conserved motif named SOC1-MOTIF near the carboxy terminus. The phylogenetic analysis showed that it might belong to SOC1/TM3 subfamily of MADS-box superfamily. The results of quantitative RT-PCR revealed that the expression level of HpSOC1 gene in stem, stem bud and flower bud was significantly higher than that of root, petal and peel. Moreover, the transcription of the HpSOC1 gene was significantly induced by 8 d night-breaking treatment, compared to untreated samples. It was deduced that HpSOC1 may play an important role in flowering of pitaya.

Key words: pitaya (Hylocereus polyrhizus); photoperiod; SOC1 homologous gene; cloning; gene expression